In-Depth Guide

Human PBMC

Understanding and Utilizing the Cornerstone Cells for Immune System Research

Peripheral blood mononuclear cells play an essential role in immunology, toxicology, inflammation, vaccination, and other research into the human immune response. As a cornerstone of discovery, it’s important for researchers in all branches of science to have a deep understanding of PBMC.

We’ve compiled some of our most popular PBMC resources — ranging from in-depth case studies to thought-provoking blog posts to detailed research protocols — into one in-depth guide. Browse the materials below to get tips and insights from our immune cell experts.

By Anne Lodge, Ph.D.

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Using a Recall Antigen Assay as a Tool for Understanding Immunity (Case Study)

While there is no industry-accepted protocol for measuring PBMC functionality, it’s an important test that should be conducted for quality control.

We needed a reliable, reproducible way to measure the functionality of our cryopreserved PBMC, so we developed a custom assay using recall antigens to understand the in vitro activity of our cells. By testing PBMC for immune response to several different antigens over time, we intended to better understand an acceptable range of variation in recall response from PBMC samples from the same donor over time.

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What Are Irradiated PBMC? (Blog Post)

Sometimes researchers need access to the components and benefits of normal PBMC, but don’t want the cells to proliferate. That’s where irradiated PBMC come in handy.

Irradiated PBMC are normal PBMC that have been exposed to radiation from a cesium 137 source to create enough DNA damage that they can no longer divide. The irradiated cells are still alive and can produce cytokines and other soluble factors, but they cannot proliferate.

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Recall Antigen Testing (Protocol)

This is the protocol we follow when testing our PBMC for reactivity to recall antigens.

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5 Benefits of Testing with Pooled PBMC (Blog Post)

Have you considered using PBMC pooled from several donors? Learn about the benefits, including eliminating costly LAL and rabbit testing, protecting horseshoe crabs, reducing donor variability, and more.

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Publications Featuring Cellero PBMC (Research Articles)

Journal articles and discoveries using our PBMC products.

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Can I Get a Donor with a Specific Disease? (Blog Post)

Yes! We have many disease-state donors available. If you don’t see the donor specifications you need, submit a custom request and we can source it for you.

Learn more about who we consider to be “normal” donors and the types of disease-state donors we can source.

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Recall Antigen Assay vs. Antigen-Specific T Cell Assay (Blog Post)

recall antigen vs cell assay tableWe have published several detailed research protocols on our website to help you improve cell performance and optimize your experimental outputs.
Two of our protocols have garnered questions from our customers and readers. Namely, what’s the difference between the Recall Antigen Assay and the Antigen-Specific T Cell Assay?

Understand the different use cases for these two important assays and get additional research tips to help you get started.

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How Fresh Are Your PBMC? (Case Study)

Post-Thaw Cell Viability chart

Figure 1. Post-thaw viability of cells isolated and cryopreserved the same day as collection and 24 hours after collection.

When ordering human or animal blood cells for research, many scientists subscribe to the adage fresh is best. But just how viable and functional are those “fresh” PBMC you ordered after sitting out of the body for 24 hours or more?

How does the timing of isolation and cryopreservation following blood sample collection impact cell viability and functionality? See the results of our experiments designed to put this freshness myth to rest.

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CFSE Labeling (Protocol)

Labeling cells with CFSE is an elegant technique for measuring cell proliferation.

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5 Reasons to Buy Normal PBMC (Blog Post)

Human peripheral blood mononuclear cells (PBMC) are one of our most popular products among researchers in academia, pharma, and biotech.

Learn the benefits of sourcing normal human PBMC from Cellero.

Explore the Benefits
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How to Thaw, Handle, and Store Frozen Cells (Blog Post)

Thawing frozen immune cell products can be tricky if you don’t take the proper precautions. It’s best to be well versed in thawing, handling, and storage techniques before receiving your cells so you can ensure viability and function for your research. Learn how we cryopreserve our cell products and how ship them to you.

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Research-Ready PBMC (Products)

Normal PBMC, Pooled PBMC, Irradiated PBMC, and Disease-State PBMC (T1-D, SLE, RA, MS & more) — all available for overnight shipping.

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