Understanding and Utilizing the Cornerstone Cells for Immune System Research
Peripheral blood mononuclear cells play an essential role in immunology, toxicology, inflammation, vaccination, and other research into the human immune response. As a cornerstone of discovery, it’s important for researchers in all branches of science to have a deep understanding of PBMC.
We’ve compiled some of our most popular PBMC resources — ranging from in-depth case studies to thought-provoking blog posts to detailed research protocols — into one in-depth guide. Browse the materials below to get tips and insights from our immune cell experts.
Table of Contents
- Using a Recall Antigen Assay as a Tool for Understanding Immunity (Case Study)
- What Are Irradiated PBMC? (Blog Post)
- Recall Antigen Testing (Protocol)
- 5 Benefits of Testing with Pooled PBMC (Blog Post)
- Publications Featuring Cellero PBMC (Research Articles)
- Can I Get a Donor with a Specific Disease? (Blog Post)
- Recall Antigen Assay vs. Antigen-Specific T Cell Assay (Blog Post)
- How Fresh Are Your PBMC? (Case Study)
- CFSE Labeling (Protocol)
- 5 Reasons to Buy Normal PBMC (Blog Post)
- How to Thaw, Handle, and Store Frozen Cells (Blog Post)
- Research-Ready PBMC (Products)
Using a Recall Antigen Assay as a Tool for Understanding Immunity (Case Study)
While there is no industry-accepted protocol for measuring PBMC functionality, it’s an important test that should be conducted for quality control.
We needed a reliable, reproducible way to measure the functionality of our cryopreserved PBMC, so we developed a custom assay using recall antigens to understand the in vitro activity of our cells. By testing PBMC for immune response to several different antigens over time, we intended to better understand an acceptable range of variation in recall response from PBMC samples from the same donor over time.
What Are Irradiated PBMC? (Blog Post)
Sometimes researchers need access to the components and benefits of normal PBMC, but don’t want the cells to proliferate. That’s where irradiated PBMC come in handy.
Irradiated PBMC are normal PBMC that have been exposed to radiation from a cesium 137 source to create enough DNA damage that they can no longer divide. The irradiated cells are still alive and can produce cytokines and other soluble factors, but they cannot proliferate.
Can I Get a Donor with a Specific Disease? (Blog Post)
Yes! We have many disease-state donors available. If you don’t see the donor specifications you need, submit a custom request and we can source it for you.
Learn more about who we consider to be “normal” donors and the types of disease-state donors we can source.
Recall Antigen Assay vs. Antigen-Specific T Cell Assay (Blog Post)
We have published several detailed research protocols on our website to help you improve cell performance and optimize your experimental outputs.
Two of our protocols have garnered questions from our customers and readers. Namely, what’s the difference between the Recall Antigen Assay and the Antigen-Specific T Cell Assay?
Understand the different use cases for these two important assays and get additional research tips to help you get started.
How Fresh Are Your PBMC? (Case Study)
When ordering human or animal blood cells for research, many scientists subscribe to the adage fresh is best. But just how viable and functional are those “fresh” PBMC you ordered after sitting out of the body for 24 hours or more?
How does the timing of isolation and cryopreservation following blood sample collection impact cell viability and functionality? See the results of our experiments designed to put this freshness myth to rest.
How to Thaw, Handle, and Store Frozen Cells (Blog Post)
Thawing frozen immune cell products can be tricky if you don’t take the proper precautions. It’s best to be well versed in thawing, handling, and storage techniques before receiving your cells so you can ensure viability and function for your research. Learn how we cryopreserve our cell products and how ship them to you.