T Regulatory Cells

Donor #: 363
Size: 1-2M cells/vial
Price: $500.00
In Stock: 18
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Donor Details

Blood Type : OPos
Age : 69
Gender : Female
Ethnicity : Caucasian
Anticoagulant : Unknown
HLA-A : 02:01,24:02
HLA-B : 15,35
HLA-C : 03,04
HLA-DRβ 1 : 01:03,01:03
CMV : Positive

Additional Donor Information:

Donor is 5′ 5″ tall and weighs 128 lbs. She has atrial tachycardia and dislipidemia and takes medications for these. See Certificate of Analysis for the specific medications. She has seasonal allergies and is allergic to penicillin. She is also CMV positive. Her Fc gamma RIIIa genotype is F/F

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About Induced T Regulatory Cells

T regulatory cells (Tregs) are a subset of T cells capable of suppressing the activation and expansion of other T cells. Currently, the most widely accepted phenotype for Tregs is the co-expression of CD4 and CD25 with additional intracellular expression of FoxP3 and absence of CD127.

Tregs can be isolated directly from peripheral blood. Alternatively, we have generated a population of Tregs in vitro with potent regulatory activity. Now you can start with ready-to-use functional Tregs instead of trying to isolate or culture enough for your experiments.

Our Treg population can be used in an in vitro suppression of T cell proliferation directly after thawing as shown in the following experiment:

In a 48 well plate, we placed 200,000 CFSE-labeled allogeneic effector peripheral blood mononuclear cells in each well in the presence of 1 μg/mL anti-CD3 and varying number of Treg or non-Treg (BC3, a Myelin Basic Protein reactive CD4+ T cell line). Both Treg and BC3 cells were directly used in this assay upon thawing.

We incubated the plate in a 37°C, 6% CO2 humidified incubator. After 4 days of culture, we harvested individual wells and labeled them with PE-Cy5-labeled anti-CD8 monoclonal antibody. Data was acquired on a flow cytometer and the extent of proliferation (Division Index, DI) from CD8+ population was analyzed using FCS Express 4.0.