NK Cells, Negatively Selected

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Donor #: 404
Size: 05-10M cells/vial
Price: $ 850.00
In Stock: 1
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Donor Details

Blood Type : ANeg
Age : 51
Gender : Male
Ethnicity : Caucasian
Anticoagulant : Citrate
HLA-A : 02:01,03:01
HLA-B : 07,13
HLA-C : 06,07
HLA-DRβ 1 : 07,15
CMV : Positive

Additional Donor Information:

Donor is a non-smoker and is CMV positive. He takes Celexa (an SSRI anti-depressant) but is otherwise healthy. His FcgRIIIa158 genotype is F/F.

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About Natural Killer Cells

Natural killer cells are important effector cells in combating viral infections and neoplasms. Their cytotoxic activity is controlled by multiple receptors that recognize MHC class I antigens. Cells that lack the appropriate MHC class I are lysed because the inhibitory receptor is not engaged.

While we only have negatively selected NK cells in stock, we can provide positively selected NK cells upon request. The positively selected population includes CD3+ CD56+ cells that are eliminated in the negative selection process.

These histograms depict the in vitro lytic activity of NK cells from lot 3100MA16. K562 target cells were labeled with carboxyfluorescein succinimidyl ester (CFSE) to distinguish them from the effector NK cells. NK cells were thawed and added to the target cells at the indicated ratios. The co-cultures were incubated for 18 hours at 37°C, 5% CO2. Cells were then washed once and 7-AAD was added to label the dead cells. In this experiment, lysis was evident at all ratios with maximum cell killing observed at a 10:1 NK:K562 ratio.