Monocyte Derived Dendritic Cells New

Donor #: 222
Size: 1-2M cells/vial
Price: $ 550.00
In Stock: 7
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Donor Details

Blood Type : ONeg
Age : 32
Gender : Female
Ethnicity : Caucasian
Anticoagulant : Citrate
HLA-A : 02:01,02:01
HLA-B : 15,27:05
HLA-C : 02,03
HLA-DRβ 1 : 07:01,04:01
CMV : Negative

Additional Donor Information:

This donor is healthy and takes no medication. She is allergic to tree pollens and keflex. Her genotype at Fc gamma RIIIa is F/V.

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About Dendritic Cells

We generate our myeloid dendritic cells by enriching monocytes, then culturing them in the presence of IL-4 and GM-CSF. Cells are collected after 5–7 days of culture and characterized by flow cytometric analysis for expression of CD11c, CD14, CD86, HLA-DR, CD1a, CD3, and CD19. The cells are frozen as immature DC but can be stimulated to full maturation as shown in the figure below.

Dendritic Cell Maturation Using Poly I:C
Directly after thawing, we plated dendritic cells in a 24 well plate at 106 cells/2 mL/well, in X-VIVOTM 15 (immature DCs) or X-VIVOTM 15 + 20 μg/mL poly I:C (mature DCs) for 48 hours in a 37°C, 6% CO2 humidified incubator.

We harvested and analyzed DCs for the surface expression of CD40, CD86, CD54, CD80, CD83, and HLA-DR/-DP/-DQ on a FACScanTM (Becton Dickinson, San Jose, CA) flow cytometer.

Histograms representing binding of antigen-specific fluorescent-labeled antibody to immature DCs (black solid line) or mature DCs (dark red histogram), as well as binding of isotype controls (dashed lines) are shown for each surface antigen tested. This data shows an increase of expression of each of the surface antigens tested, demonstrating DC maturation after 48 hours of culture in the presence of poly I:C.

We also have a variety of mouse dendritic cells available.