The Epstein-Barr virus (EBV) is one of the most prevalent infections, found in about 95% of the adult population. EBV is a lifelong infection with ties to many diseases.

Although infected individuals often build adaptive immunity to EBV and are asymptomatic, EBV is associated with infectious mononucleosis, several types of cancer such as Hodgkin’s lymphoma, and autoimmune diseases including multiple sclerosis and HIV.

 

Studying EBV in the Immune System

Researchers are using EBV T cells to understand the interaction between the immune system and chronic infections, as well as look for cures to the various diseases caused by and associated with EBV infection.

Researchers are also using EBV to aid in understanding tumor immunology. Because EBV is associated with a variety of malignant tumors, EBV T cells are playing an important role in the development of immunotherapies.

 

Interested in Studying Epstein-Barr?

Astarte now offers an EBV specific T cell line that produces IFNg when stimulated with an EBV-derived peptide bound to HLA-A*0201. In addition to our EBV T cells, we also offer antigen-presenting cells and an EBV-derived peptide — everything you need to get started with your EBV experiments.

 

EBV T Cell Assay

Materials

  • EBV T Cells
  • B-LCLs
  • EBV Peptide
  • Culture Medium (e.g. X-VIVO 15)

Procedure

  1. Add B-LCL t0 96-well U-bottom plate at 20,000 cells per well
  2. Add EBV peptide at range of concentrations
  3. Add EBV T cells at 20,000 cells per well
  4. Incubate at 37°C with 5% COfor 1 day
  5. Collect culture medium and measure cytokine production

Leave a Reply

Your email address will not be published. Required fields are marked *

Post comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.