Activation of immune cells is the all-important first step in mounting an immune response. Immune cell activation is a popular area of research because so much happens that is key to the downstream goal of fighting infection, cancer and disease.

There are many ways to measure immune cell activation, and they all have utility. Methods can be grouped into four main categories: Proliferation Assays, Cytokine Measurement, Surface Antigen Expression and Cytotoxicity.

Webinar: 14 Ways to Measure Immune Cell Activation

On Tuesday, September 18th at 8:00 am PT (11:00am ET), we are presenting a webinar, hosted by Bitesize Bio, covering the joys and pitfalls of several specific assays along with recommendations on how to choose the best methods for your research needs.

Join our Chief Scientific Officer, Anne Lodge, as she shares specific tips and strategies for successful assay development using the following methods.

Examples of ATP Luminescence and Bromodeoxyuridine (BrdU) Uptake


  • 3H-Thymidine Uptake
  • Bromodeoxyuridine Uptake (BrdU)
  • ATP Luminescence
  • Fluorescent Dye Reduction (CFSE)

Cytokine Measurement

  • Multiplex vs. Single Cytokine
  • Choice of Cytokine (IFNg, TNFa, IL-6, IL-1β, etc.)
  • Kinetics of Cytokine Release

Surface Antigen Expression

  • CD69, CD25, PD-1, etc.
  • Combine with CFSE, Ki67 or BrdU
  • Kinetics are Important


  • Two-Label Flow Cytometry
  • Calcein AM Dye Release
  • Luciferase Transduced Targets
  • Annexin V

We hope to see you there! Stay on after the presentation for a live Q&A session.

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