Activation of immune cells is the all-important first step in mounting an immune response. Immune cell activation is a popular area of research because so much happens that is key to the downstream goal of fighting infection, cancer and disease.
There are many ways to measure immune cell activation, and they all have utility. Methods can be grouped into four main categories: Proliferation Assays, Cytokine Measurement, Surface Antigen Expression and Cytotoxicity.
Webinar: 14 Ways to Measure Immune Cell Activation
On Tuesday, September 18th at 8:00 am PT (11:00am ET), we are presenting a webinar, hosted by Bitesize Bio, covering the joys and pitfalls of several specific assays along with recommendations on how to choose the best methods for your research needs.
- 3H-Thymidine Uptake
- Bromodeoxyuridine Uptake (BrdU)
- ATP Luminescence
- Fluorescent Dye Reduction (CFSE)
- Multiplex vs. Single Cytokine
- Choice of Cytokine (IFNg, TNFa, IL-6, IL-1β, etc.)
- Kinetics of Cytokine Release
Surface Antigen Expression
- CD69, CD25, PD-1, etc.
- Combine with CFSE, Ki67 or BrdU
- Kinetics are Important
- Two-Label Flow Cytometry
- Calcein AM Dye Release
- Luciferase Transduced Targets
- Annexin V
We hope to see you there! Stay on after the presentation for a live Q&A session.