Nitric oxide (NO) was the first gas shown to impact normal bodily functions, including the immune system, earning it the Science Molecule of the Year award in 1992. In addition to the immune system, small amounts of NO in the body have also demonstrated effects on blood pressure, long-term memory, the nervous system, and several organs, making the measurement of NO important to several disciplines.

Other NO kits require a Griess reagent in multiple components. We’ve simplified our Nitric Oxide Assay Kit so you can more easily measure NO production for your immune system research. Detailed instructions included.

How It Works

We currently have a Nitric Oxide Assay Kit for mouse cells and will be releasing the human cell version soon.
Culture medium from mouse cells will have nitrites derived from the NO. We’ve seen higher NO when the culture medium is collected 48 hours after stimulation of the macrophages. Cells need to be “primed” to make NO. Our mouse dendritic cells will make NO when stimulated with LPS.

In human cultures, the nitrite is further oxidized to nitrate. To measure nitrate, we will include nitrate reductase in the human kit.

What’s Included

Mouse Nitric Oxide Assay Kit

  • Griess reagent, 100 mL
  • 1 mM Sodium Nitrite for use as a standard, 30 mL

Human Nitric Oxide Assay Kit

  • Griess reagent, 100 mL
  • 1 mM sodium nitrite, 30 mL
  • 1 mM sodium nitrate, 30 mL
  • Nitrate reductase
  • Enzyme cofactors

 
One Nitric Oxide Assay Kit is good for 960 assays.
 

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