Using PBMC as a Quality Control for Flow Cytometry

Our cryopreserved peripheral blood mononuclear cells are a great tool for flow cytometry assays. Whether you want to confirm your antibodies are working properly or measure for consistency, our PBMC are the tools you need for quality control.  Using PBMC to Test Antibodies Sometimes you might not be sure if your chosen reagent is correctly…

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Ask A Scientist: Proteoglycans vs. Glycoproteins

The extracellular matrix is a complex combination of non-cellular materials that support biochemical and biomechanical processes in the tissues and organs. Among the components of the extracellular mix are two distinct types of biomolecules: proteoglycans and glycoproteins. Immunology researchers are primarily interested in these biomolecules due to their role in the human tissue processes and…

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In-Depth Guide for Working with Macrophages

Macrophages have many applications in immunology research and can produce a wide range of cytokines and chemokines that can influence the immune response in both mouse and human models.  We’ve compiled an in-depth guide with our best resources for working with macrophages, the “Big Eaters” of the immune system. You’ll get tips for creating and…

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How to Differentiate Monocytes into Osteoclasts

Our contract immunology research team is always looking for a new challenge. So when a customer proposed a project that involved differentiating monocytes into osteoclasts, we said, “Why not!” Fun in the Lab with Monocytes Osteoclasts are macrophages found in bone and are highly specialized myeloid cells that break down and resorb bone tissue, important…

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Endotoxin Detection: How Clean Are Your Antigens?

Have you ever spent weeks or months pursuing a mysterious artifact in your culture only to discover that the cause was a reagent or culture media contaminated with bacterial endotoxin? You’re not alone. Continue reading for a cautionary tale on endotoxin contamination. Detecting a Problem: Clueing in on Endotoxin Contamination We encountered an endotoxin contamination…

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New CMV-Specific CD4+ T Cells

New to our ever-expanding immune cell inventory is a CMV-Specific CD4+ T Cell Line, available in large batches of 1–2M cells/vial. Our CMV-specific T cells have been extremely popular among our customers for years, but have only been available as CD8+ cell lines until now. As immunologists, we naturally think of CD8+ T cells when…

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