We have created several different T cell lines specific for tumor-associated antigens, and now have developed and validated a cell-mediated cytotoxicity assay to see if the tumor antigens are presented by tumor cells to the cytotoxic T cells. Using the IncuCyte® S3 to Image Co-Cultures and Observe Cell Killing In this assay, we used…
For some scientists, the difference between serum and plasma is just nomenclature. On more than once occasion we’ve had to ask customers, “Do you mean serum or do you mean plasma?” only to hear, “either one” or “aren’t they the same thing?” Let us clear up the confusion. Serum vs. Plasma: What’s the Difference?…
Although organ transplantation has added millions of life-years to recipients and advanced considerably since the 1980s, one-year survival rates for solid-organ transplants have plateaued in recent years, and long-term survival rates have not changed much since the ‘80s.1 Research into the immune system’s role in organ transplantation success is critical to improving these rates, particularly…
Transferring immune cell products from the freezer to shipment containers and eventually to the end user’s freezer involves big temperature swings and potential stress on the cells. With many years of experience as an immune cell provider shipping products worldwide, we have perfected our temperature control and shipping processes. Our standard shipping methods will mitigate…
Peptide-MHC tetramer staining is a common practice with both R&D and clinical applications in infectious disease investigations, vaccine development, immunotherapy and autoimmunity research, and transplantation. Experimentally, this method can be used to measure detailed T cell responses and functions. Clinically, peptide-MHC tetramer staining is used to count, track, and phenotype T cells during infections and…
How Many Cells Are in a Leukopak? Our leukapheresis products typically contain 5 to 20 billion white blood cells, depending on the volume of blood processed from the donor during leukapheresis. In addition to white blood cells, our leukopaks also contain plasma, some platelets, and very few red blood cells. The table below explains the…
Cellero’s scientists bring a wealth of immunology research expertise from diverse backgrounds. With extensive experience in a variety of biologics disciplines, our team is an ideal resource for a wide range of outsource research services. From donor collection to flow cytometric analyses, Cellero helps you reach your milestones. In this Researcher Spotlight, we introduce you…
Type 1 diabetes affects approximately 1.25 million Americans today, and that number is expected to rise dramatically to 5 million by 2050. In addition to its own health complications, diabetes can be a dangerous and deadly comorbidity for patients exposed to viruses, diseases, or other health challenges. As we have seen with the COVID-19 pandemic,…
Macrophages are a diverse group of white blood cells known for eliminating pathogens through phagocytosis. In the past, macrophages were classified by the organ in which they were found: Kuppfer cells in the liver, Langerhans cells in the skin, microglia in the brain and spinal cord, osteoclasts in the bone. Scientists continue to debate whether…
How Do You Culture and Expand T Cells, PBMC, and Other Immune Cells? We get these types of culture questions all the time. After all, we developed the immune cells, have tested their functionality, and created research protocols specific to them. So naturally, we can help! However, the answers to these questions aren’t always straightforward.…