With so many commercial characterized immune cell, leukapheresis, and apheresis products available in one place, it can be difficult to determine the best fit for your research and development needs.

The big decisions immune cell and biomaterial buyers face comes down to two factors:

  • Fresh or frozen product?
  • By the vial or full leukopak?

This mini-immune cell buyer’s guide will give you the tools to make the right decision. If you still have questions, ask a Cellero scientist and get a quick response! We’re happy to help.

The Quick Decision-Making Guide for Immune Cell Buyers

Fresh or Frozen?

Some scientists are adamant that “fresh is always best” — and maybe you’re one of them! One important thing to consider is, how fresh is fresh?

With our fresh leukapheresis products, platelets, and whole blood, you never have to worry about freshness. Once we confirm your order details and collection schedule, we package and ship your product fresh overnight to ensure viability. In some locations, we can offer same day delivery. We also offer continuous temperature monitoring devices to ensure the temperature during shipment stays within the parameters of the protocol.

When it comes to characterized immune cells, you have to question your supplier on the length of time between collection and cryopreservation. Have the cells been in transit to the lab for 18 hours? 24 hours? Longer?

Cells frozen the same day as collection are going to outperform cells that were frozen after overnight shipment in the true measures of cell quality: viability and functionality.

 

Related Case Study: How Fresh Are Your PBMC?
How timing of isolation and cryopreservation following blood sample collection impact cell viability and functionality.

 

Also consider your desired application. Are you developing an assay that will be used on clinical trial samples that are shipped overnight? Don’t develop the assay with cells that are same-day fresh. Use samples that resemble the eventual real-world conditions.

Our frozen characterized immune cells are isolated and cryopreserved on the same day as collection, typically within 3 hours of arriving at our lab. We offer cells frozen in different concentrations so you can thaw just what you need for your experiment. Our cells are shipped on dry ice at about –79°C to maintain a consistent temperature.

Our frozen leukopaks can be collected using our proprietary protocols or a sponsor’s protocol and are ideal for research and development as well as clinical applications. Going with frozen leukopaks instead of fresh gives you flexibility in scheduling your processes with a ready-to-thaw option.

Vial or Leukopak?

Cells by the vial are ideal for experiments that require running the same cells multiple times. These samples provide the best material for limiting the variation from one blood draw to the next. Plus, the convenience is a time-saver for any busy scientist.

You can purchase multiple vials in bulk from the same donor and lot, ensuring the materials in your experiments are consistent. We typically post new products with dozens of vials available in the same lot. We also offer PBMC from the same donors in multiple vial sizes so you can choose the perfect fit for your experiment without any waste.

If you need a steady supply of effector cells as raw materials in research and development, leukopaks are the best route. Leukopaks are generally collected from healthy donors and shipped fresh overnight, containing between 5 and 20 billion white blood cells depending on the volume of blood processed from the donor during leukapheresis. We can collect non-mobilized and mobilized leukopaks in various sizes and to your exact specifications.

High-volume leukopaks may be beneficial to your research if you are:

  • Conducting late-stage immunology research
  • Developing cell-based therapies
  • Perfecting the cellular therapy manufacturing process (gene insertion, cell maturation, differentiation, confirmation of reproducibility)
  • Validating processes and training production staff
  • Looking for a large amount of cells from one donor
  • Planning to consume a high volume of cells at one time

 

Still Can’t Decide?

Contact us to discuss the best options for your research.

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