If you haven’t noticed already, our collection of antigen-specific T cells is growing quickly, specifically in the family of autoimmune diseases!

Our current available inventory includes T cells that are specific for antigens believed to be responsible for tissue damage in multiple sclerosis, diabetes, and vitiligo. Learn how you can apply these unique cells to your research.

 

Better Understand Multiple Sclerosis

Myelin basic protein (MBP) is a very well-characterized human antigen, but also one of the most complex. Changes in MBP structure have been studied closely as a cause of demyelination in multiple sclerosis.

Although human T cells have been shown to recognize many MBP epitopes using various HLA molecules, the peptide we used to develop our MBP-specific T cell line (ENPVVHFFKNIVTPRTP) is widely believed to be the immunodominant region of the protein.

MBP-specific T cells are found in the circulation of both healthy humans and those with multiple sclerosis. The donor for our MBP-specific T cell line is a healthy Caucasian 56-year-old male.

These T cells are CD4+, will proliferate when they encounter the peptide bound by HLA-DRβ1*15:01, and will also secrete IFNγ, IL-13, and TNFα. TCR sequencing shows this to be a clonal cell line.

Related: MBP Peptide

 

 

Move the Needle on Diabetes Research

Enabling diabetes research has been a major focus in our lab over the past two years. We now have four antigen-specific T cell lines for diabetes researchers: two that respond to proinsulin and two for GAD65.

Proinsulin-Specific T Cells

Of our two proinsulin-specific T cell lines, the PI-1 line behaves more like a Th1 intracellular defense secreting higher levels of IFNγ and lower levels of IL-5 and IL-13 while the other line for PI-3 behaves more like a Th2 extracellular defense with higher IL-5 secretion. Both lines recognize peptides presented by HLA-DRβ1*14:01.

The donor for both lines is a healthy Caucasian 32-year-old female.

Related: Proinsulin 73–90 Peptide

 

GAD65-Specific T Cells

The GAD65 glutamic acid decarboxylase 65-kilodalton isoform is the first antigen to be recognized in diabetic subjects, making the GAD65 antibodies an extremely important marker for the development of type 1 diabetes and other nonneurological central nervous system autoimmune diseases.

Our two CD4+ T cell lines for this epitope again behave more Th1-like (lot 4642) and more Th2-like (lot 4645). The donor for both lines is a healthy Hispanic 48-year-old male.

 

Explore Vitiligo Causes and Treatments

You might associate our MART-1-specific T cells with melanoma research, but the MART-1 antigen has also been studied in connection with vitiligo, a skin pigmentation disorder. MART-1 is expressed by normal melanocytes (skin pigment cells), which classifies it as an autoantigen.

Our MART-1 T cell line consists of CD8+ T cells developed by repeated stimulation with the naturally occurring MART-1 peptide. These versatile cells also recognize a peptide that has been slightly altered to improve binding to the HLA-A*02:01 molecule.

Related: MART-1 Peptide

 

If you’re working on a particular autoimmune disease, talk to us about developing a custom cell line for your research.

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